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Image Search Results
Journal: Scientific Reports
Article Title: Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells
doi: 10.1038/s41598-017-01627-1
Figure Lengend Snippet: Cell surface antigen analysis by two-color flow cytometry. ( a ) Phenotype analysis of lymphocytes of the lymph node by flow cytometric analysis with a combination of anti-CD3/-CD20 and anti-CD27/-CD20 antibodies. B cells are surrounded by a dotted line. ( b ) Phenotype analysis of B cells purified using CD19-microbeads by flow cytomeric analysis with a combination of anti-CD3/-CD20 antibodies. Purified B cells are surrounded by a dotted line.
Article Snippet: B cells were purified using magnetic-activated
Techniques: Flow Cytometry, Purification
Journal: Scientific Reports
Article Title: Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells
doi: 10.1038/s41598-017-01627-1
Figure Lengend Snippet: Characterization of the BiPSCs. ( a ) Immunofluorescence staining of BiPSC13 and MIB2-6 for expression of the pluripotent markers Oct3/4, Nanog, SSEA4, TRA-1-60, and TRA-1-81. ( b ) Expression of endogenous Oct3/4, Sox2, Klf4, cMyc, Pax5, AID , and GAPDH in BiPSCs (BiPSC13, MIB2-6) and normal B cells (CD19) from the lymph node analyzed using RT-PCR. ( c ) RT-PCR analysis of the expression of retrovirus-derived Oct3/4, Sox2, Klf4 , and cMyc in BiPSCs (BiPSC13, MIB2-6). HUC-Fm, human umbilical cord fibroblast cells, (male; RIKEN, Tsukuba, Japan) infected with a retrovirus containing Oct3/4 , Sox2 , Klf4 , c-Myc , and GAPDH for 5 days were used as the positive control.
Article Snippet: B cells were purified using magnetic-activated
Techniques: Immunofluorescence, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Infection, Positive Control
Journal: Scientific Reports
Article Title: Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells
doi: 10.1038/s41598-017-01627-1
Figure Lengend Snippet: AID expression in BiPSCs induced with the doxycycline-controlled (Tet-off) system. ( a ) Induction of AID expression in the absence of doxycycline was confirmed in two clones (#1 and #2) derived from BiPSC13 by western blotting, and ( b ) qRT-PCR. In ( b ), the numbers on the Y axis are the relative ratio comparing the expression of AID mRNA of each sample standardized by the expression of GAPDH mRNA with that of CD19 + normal B cells purified from normal LN using CD19-microbeads. Raji, Burkitt lymphoma cell line. Data were analyzed in triplicates and normalized to glyceraldehyde 3-phosphate dehydrogenase. ( c ) Immunofluorescence analysis of the expression of Oct3/4 and Nanog in BiPSC13-AID (#1 and #2) after the induction of AID expression in the absence of doxycycline. ( d ) Induction of AID expression in the absence of doxycycline in two clones (#16 and #17) derived from MIB2-6 by western blotting, and ( e ) qRT-PCR as described in the legend to ( b ). ( f ) Immunofluorescence analysis of the expression of Oct3/4 and Nanog in MIB-AID (#16 and #17) after the induction of AID expression in the absence of doxycycline.
Article Snippet: B cells were purified using magnetic-activated
Techniques: Expressing, Clone Assay, Derivative Assay, Western Blot, Quantitative RT-PCR, Purification, Immunofluorescence